Fluorescence in situ hybridisation (FISH) is a recently
developed molecular cytogenetic technique, involving
hybridisation of a DNA probe to a metaphase chromosome
spread. Single stranded probe DNA is fluorescently labelled
using biotin and avidin and hybridised to the denatured DNA
of intact chromosomes on a microscope slide. The resultant
DNA binding can be seen directly using a fluorescence
microscope.
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